Scheuring Lab

Looking at plants from a cell´s perspective.

Research

Cells are the smallest units of life. Due to their sessile lifestyle, plants rely in particular on intracellular reactions to cope with changing environmental conditions. Thus, our research focus is to understand how plant cells behave during stress situations.
We aim to understand the cellular and molecular processes which allows for cell elongation and eventually plant growth. To this end, we use a combination of cell biological, genetic and biochemical approaches. Especially vesicle transport along actin filaments and morphological changes of cell organelles (e.g. the vacuole) are being investigated during abiotic and biotic (pathogens) stress. Deciphering these specific intracellular processes will allow to understand stress-induced plant growth regulation which might help to establish more robust plants withstanding future challenges. 

Our team

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David Scheuring
Principle Investigator

Organizing, administrating and structuring different projects

Curriculum Vitae

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Sabrina Kaiser
PhD candidate

Investigating the connection between the plant cytoskeleton and the vacuole

kaisers@rptu.de

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Jonas Müller
PhD candidate

Deciphering the salicylic acid induced plant growth inhibition



jonasmue@rptu.de

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Tobias Müller
PhD candidate

Understanding how plant defense is manipulated by pathogens



tmueller@rptu.de 

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Paulina Miranda Ramirez
Master Student
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Yvonne König
Bachelor Student
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Tanaz Azizi
Student Helper
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Publications

Original research: first –and corresponding authorships from our group

T Mueller, J Bronkhorst, J Mueller, N Safari, M Hahn, J Sprakel, Scheuring, D (2023) Plant infection by the necrotrophic fungus Botrytis requires actin-dependent generation of high invasive turgor pressure. bioRxiv, https://doi.org/10.1101/2023.12.15.571920

Kaiser S, Mehlhorn D, Ramirez Miranda P, Ries F, Sommer F, Schroda M, Schumacher K, Willmund F, Grefen C, Scheuring, D (2023) Networked proteins redundantly interact with VAP27 and RABG3 to regulate membrane tethering at the vacuole and beyond. bioRxiv, https://doi.org/10.1101/2023.09.29.56011

Jeblick T, Leisen T, Steidele CE, Albert I, Mueller J, Kaiser S, Mahler F, Sommer F, Keller S, Hueckelhoven R, Hahn M, Scheuring, D (2023) Botrytis hypersensitive response inducing protein 1 triggers non-canonical PTI to induce plant cell death. Plant Physiol, 191, 125-141

Kaiser S, Eisele S, Scheuring D (2021) Vacuolar occupancy is crucial for cell elongation and growth regardless of the underlying mechanism. Plant Signal. Behav. 16, 1922796

Kaiser S, Eisa A, Kleine-Vehn J, Scheuring D. (2019) NET4 modulates the compactness of vacuoles in Arabidopsis thaliana. Int. J. Mol. Sci, 20, 4752

Scheuring D, Löfke C, Krüger F, Kittelmann M, Eisa A, Hughes L, Smith RS, Hawes C, Schumacher K, Kleine-Vehn J. (2016) Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression. Proc Natl Acad Sci U S A. 113, 452-57

Löfke C, Scheuring D, Dünser K, Schöller M, Luschnig C and Kleine-Vehn J. (2015) Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth. J Exp Bot. 66, 5103-5112

Scheuring D, Künzl F, Viotti C, San Wan Yan M, Jiang L, Schellmann S, Robinson D.G and Pimpl P. (2012) Ubiquitin initiates sorting of Golgi and plasma membrane proteins into the vacuolar degradation pathway. BMC Plant Biol. 12, 164

Scheuring D, Viotti C, Krüger F, Künzl F, Sturm S, Bubeck J, Hillmer S, Frigerio L, Robinson D.G, Pimpl P and Schumacher K. (2011) Multivesicular bodies mature from the trans-Golgi network/early endosome in Arabidopsis. Plant Cell 23, 3463-81

Bubeck J, Scheuring D, Hummel E, Langhans M, Viotti C, Foresti O, Denecke J, Banfield D.K and Robinson D. G. (2008) The syntaxins SYP31 and SYP81 control ER Golgi trafficking in the plant secretory pathway. Traffic 9, 1629-52

Review articles, book chapters and commentaries

Scheuring D and Kleine-Vehn J. (2020) On the discovery of an endomembrane compartment in plants. Proc Natl Acad Sci U S A. 117, 10623-10624

Kaiser S and Scheuring D. (2020) To lead or to follow: Contribution of the plant vacuole to cell growth. Front. Plant Sci. 11, 553

Ruano G and Scheuring D. (2020) Plant Cells under Attack: Unconventional Endomembrane Trafficking during Plant Defense. Plants. 21, 389

Scheuring D, Schöller M, Kleine-Vehn J and Löfke C. (2015) Vacuolar staining methods in plant cells. Methods Mol Biol. 1242, 83-92

Scheuring D and Kleine-Vehn J. (2014) Intracellular auxin transport. Springer press: Auxin and Its Role in Plant Development. pp 61-73. DOI: 10.1007/978-3-7091-1526-8_4

Robinson DG, Pimpl P, Scheuring D, Stierhof YD, Sturm S, Viotti C. (2012) Trying to make sense of retromer. Trends Plant Sci. 17, 431-9

Robinson DG, Scheuring D, Naramoto S and Friml J. ARF1 Localizes to the Golgi and the Trans-Golgi Network. (2011) Plant Cell 23, 846-9 

Collaborations

Kreis E, Niemeyer J, Merz M, Scheuring D, Schroda M (2023) CLPB3 is required for the removal of chloroplast protein aggregates and for thermotolerance in Chlamydomonas. J. Exp. Bot. doi: 10.1093/jxb/erad109

Kuang L, Chen S, Guo Y, Scheuring D, Flaishman MA, Ma M (2022) Proteome analysis of vacuoles isolated from fig (Ficus carica L.) flesh during fruit development. Plant Cell Physiol. pcac039

Leisen T, Werner J, Pattar P, Safari N, Ymeri E, Sommer F, Schroda M, Suárez I, Collado IG, Scheuring D, Hahn M (2022) Multiple knockout mutants reveal a high redundancy of phytotoxic compounds that determine necrotrophic pathogenesis of Botrytis cinerea. PLoS pathogens 18 (3), e1010367

Trösch R, Ries F, Westrich LD, Gao Y, Herkt C, Hoppstädter J, Heck-Roth J, Mustas M, Scheuring D, Choquet Y, Räschle M, Zoschke R, Willmund, F (2021) Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation. The Plant Cell, koab317, https://doi.org/10.1093/plcell/koab317

Valifard M, Le Hir R, Müller J, Scheuring D, Neuhaus HE, Pommerrenig B (2021) The vacuolar fructose transporter SWEET17 is critical for root development and drought tolerance. Plant Phys. 187, 2716–2730

Hickl D, Drews F, Girke C, Zimmer D, Mühlhaus T, Hauth J, Nordström K, Trentmann O, Neuhaus HE, Scheuring D, Fehlmann T, Keller A, Simon M, Möhlmann T (2021) Differential degradation of RNA species by autophagy related pathways in Arabidopsis. J. Exp. Bot. 72, 6867-6881

Minina EA, Scheuring D, Askani J, Krueger F, Schumacher K (2021) Light at the end of the tunnel: FRAP assay reveals that plant vacuoles start as a tubular network. bioRxiv https://www.biorxiv.org/content/10.1101/2021.05.13.444058v1

Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M (2021) Real-time monitoring of subcellular H2O2 distribution in Chlamydomonas reinhardtii. Plant Cell, 33, 2935-2949

DJ Klionsky et al. (2021) Guidelines for the use and interpretation of assays for monitoring autophagy. autophagy. 17, 1-382

Hickl D, Scheuring D, Möhlmann T (2021) CTP-Synthase 2 from Arabidopsis thaliana is required for complete embryo development. Front. Plant Sci. 12, 510

Leisen T, Bietz F, Werner J, Wegner A, Schaffrath U, Scheuring D, Willmund F, Mosbach A, Scalliet G, Hahn, M. (2020) CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea. PLOS Pathogens 16: e1008326

Müller N, Leroch M, Schumacher J, Zimmer D, Könnel A, Klug K, Leisen T, Scheuring D, Sommer F, Mühlhaus T, Schroda M, Hahn M. (2018) Investigations on VELVET regulatory mutants confirm the role of host tissue acifidication and secretion of proteins in the pathogenesis of Botrytis cinerea. New Phytol. 219, 1062-1074

Löfke C, Dünser K, Scheuring D and Kleine-Vehn J. (2015) Auxin regulates SNARE-dependent vacuolar morphology restricting cell size. eLife 4, e05868

Viotti C, Krüger F, Krebs M, Neubert C, Fink F, Lupanga U, Scheuring D, Boutté Y, Frescatada-Rosa M, Wolfenstetter S, Sauer N, Hillmer S, Grebe M and Schumacher K. (2013) The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis. Plant Cell 25, 3434-49

Stierhof YD, Viotti C, Scheuring D, Sturm S and Robinson DG. (2013) Sorting nexins 1 and 2a locate mainly to the TGN. Protoplasma 250, 235-40

Lerich A, Hillmer S, Langhans M, Scheuring D, van Bentum P and Robinson DG. (2012) ER Import Sites and Their Relationship to ER Exit Sites: A New Model for Bidirectional ER-Golgi Transport in Higher Plants. Front Plant Sci. 2, 143

Shahriari M, Keshavaiah C, Scheuring D, Sabovljevic A, Pimpl P, Hausler R.E, Hulskamp M and Schellmann S. (2010) The AAA-ATPase AtSKD1 contributes to vacuolar maintenance of A. thaliana. Plant J 64, 71-85

Niemes S, Labs M, Scheuring D, Krueger F, Langhans M, Jesenofsky B, Robinson DG and Pimpl P. (2010) Sorting of plant vacuolar proteins is initiated in the ER. Plant J 62, 601-14

Niemes S, Langhans M, Viotti C, Scheuring D, San Wan Yan M, Jiang L, Hillmer S, Robinson D.G and Pimpl P. (2010) Retromer recycles vacuolar sorting receptors from the trans-Golgi network. Plant J 61, 107-21 

Contact

PD Dr. David Scheuring

Plant Pathology
University of Kaiserslautern (RPTU)
Paul-Ehrlich-Str. 22
67663 Kaiserslautern, Germany

Email: scheuring@uni-kl.de
Phone: +49 (0)631 205-2219

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